Coding
Part:BBa_K258002:Design
Designed by: Ozkan IS Group: iGEM09_METU-Gene (2009-10-03)
Granulysin, a T Cell Product,Kills Bacteria by Altering Membrane Permeability
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 289
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Granulysin can be purified via nickel affinity chromatography.
Source
Granulysin is a member of the saposin-like protein (SAPLIP) family. The gene was synthesized at GENEART in pMA vector with standart Biobrick restriction sites
References
William A. Ernst, Sybille Thoma-Uszynski, Rachel Teitelbaum,Christine Ko,Dennis A. Hanson, Carol Clayberger, Alan M. Krensky, Matthias Leippe, Barry R. Bloom, Tomas Ganz,Robert L. Modlin.Granulysin, a T Cell Product, Kills Bacteria by Altering Membrane Permeability. The Journal of Immunology, 2000, 165: 7102–7108.